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American Society of Microbiology ABMM Exam Syllabus Topics:

SectionObjectives
Topic 1: Clinical Microbiology- Parasitology
  • 1. Human parasitic infections
    • 2. Diagnostic techniques in parasitology
      - Mycology
      • 1. Laboratory identification of fungi
        • 2. Medical fungal infections
          - Virology
          • 1. Diagnostic virology methods
            • 2. Clinical viral pathogens
              - Bacteriology
              • 1. Antimicrobial susceptibility testing principles
                • 2. Identification and classification of bacterial pathogens
                  Topic 2: Laboratory Management & Quality Systems- Regulatory compliance
                  • 1. Clinical laboratory regulations and accreditation standards
                    • 2. Safety and biosafety requirements
                      - Quality assurance and quality control
                      • 1. Proficiency testing and validation
                        • 2. Laboratory quality systems design
                          Topic 3: Infectious Disease Principles- Epidemiology
                          • 1. Transmission and outbreak investigation
                            • 2. Public health microbiology
                              - Pathogenesis
                              • 1. Mechanisms of microbial infection
                                • 2. Host-pathogen interactions
                                  Topic 4: Laboratory Operations- Instrumentation and methodologies
                                  • 1. Molecular diagnostic techniques
                                    • 2. Automated microbiology systems
                                      - Diagnostic workflow
                                      • 1. Test selection and interpretation
                                        • 2. Specimen handling and processing

                                          American Society of Microbiology ABMM Sample Questions:

                                          1. A clinical microbiology laboratory is preparing to validate a new molecular assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA). To assess the positive predictive value (PPV) of the assay, they need to know:

                                          A) The number of true negative results divided by the total number of true negative and false positive results.
                                          B) The number of true positive results divided by the total number of true positive and false negative results.
                                          C) The number of true negative results divided by the total number of negative test results.
                                          D) The number of true positive results divided by the total number of positive test results.


                                          2. A microbiology laboratory is asked to identify the causative agent of a fungal keratitis. Microscopic examination of corneal scrapings reveals broad, aseptate hyphae with right-angle branching. The MOST likely genus is:

                                          A) Mucor or Rhizopus
                                          B) Candida
                                          C) Aspergillus
                                          D) Fusarium


                                          3. A patient with a history of travel to an area endemic for schistosomiasis presents with hematuria. Microscopic examination of a urine sediment reveals a large oval egg with a prominent terminal spine. The MOST likely causative agent is:

                                          A) Schistosoma haematobium
                                          B) Schistosoma japonicum
                                          C) Schistosoma mansoni
                                          D) Schistosoma mekongi


                                          4. A patient with a history of intravenous drug use develops a fever and new heart murmur. Blood cultures are positive for Gram-positive cocci that are catalase-positive and coagulase-positive. The MOST likely complication of this infection is:

                                          A) Reactive arthritis
                                          B) Septic arthritis
                                          C) Glomerulonephritis
                                          D) Embolic stroke


                                          5. A clinical microbiology laboratory is performing antimicrobial susceptibility testing using the broth microdilution method. The minimum bactericidal concentration (MBC) is determined by:

                                          A) The lowest concentration of antibiotic that inhibits visible growth after 24 hours of incubation.
                                          B) Subculturing the clear wells from the MIC assay onto antibiotic-free media and determining the lowest concentration that results in no bacterial growth.
                                          C) Comparing the zone of inhibition around antibiotic disks to standardized tables.
                                          D) The highest concentration of antibiotic that allows visible growth after 24 hours of incubation.


                                          Solutions:

                                          Question # 1
                                          Answer: D
                                          Question # 2
                                          Answer: A
                                          Question # 3
                                          Answer: A
                                          Question # 4
                                          Answer: D
                                          Question # 5
                                          Answer: B

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